Improving wound healing function and storage stability of stem cell-derived extracellular vesicles via lyophilized hyaluronic acid formulation - PubMed
11 hours ago
- #Wound healing
- #Hyaluronic acid
- #Extracellular vesicles
- The study presents a lyophilized hyaluronic acid (HA)-coated formulation of extracellular vesicles (EVs) derived from human adipose stem cells to enhance therapeutic efficacy and storage stability.
- HA-EVs leverage the natural affinity between HA and CD44 proteins on EV membranes, forming stable complexes that remain intact after lyophilization and rehydration.
- HA-EVs show superior targeting of CD44-expressing cells, including pro-inflammatory macrophages and senescent dermal fibroblasts, suppressing inflammation and restoring fibroblast function in wound healing.
- In a mouse wound-healing model, HA-EVs significantly accelerated wound closure by promoting collagen synthesis in the dermal layer, outperforming bare EVs.
- HA acts as an effective cryoprotectant, preserving EV integrity for at least six months at 4°C, making it suitable for long-term storage.
- The formulation integrates cell-specific targeting with storage stability, offering a clinically translatable platform for regenerative medicine.