An alkaline nanosized platelet vesicle-based hydrogel for the treatment of osteoporotic bone defects - PubMed

6 hours ago

Osteoporosis is a systemic bone disease characterized by excessive osteoclast differentiation, leading to reduced bone mass and increased fragility.
Alkalinizing lysosomes is a potential therapeutic strategy for osteoporotic bone defects due to the low lysosomal pH required by overactive osteoclasts.
Nanosized platelet vesicles (NPVs) loaded with sodium bicarbonate (NaHCO3@NPVs) were developed for repairing osteoporotic bone defects.
The NaHCO3@NPVs were encapsulated in an extracellular matrix hydrogel (NaHCO3@NPVs-EH) made from fibrinogen and thrombin.
The hydrogel releases NaHCO3@NPVs at the defect site, which are internalized by bone marrow-derived macrophages (BMDMs).
NaHCO3@NPVs inhibit osteoclast differentiation and activity through a 'biochemical dual-action' mechanism: alkalinizing lysosomes and depleting lysosomal calcium, and bioactive factors in NPVs directly or indirectly inhibiting osteoclasts.
In vivo experiments in a rat model showed that NaHCO3@NPVs-EH reduced overactive osteoclasts and enhanced bone repair.
The study suggests NaHCO3@NPVs-EH as a promising treatment for osteoporotic bone defects and introduces a new acid-base neutralization perspective for osteoporosis treatment.

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