Oxidized PDI promotes thrombus formation in oxidative stress - PubMed
5 days ago
- #oxidative stress
- #protein disulfide isomerase
- #thrombus formation
- Oxidized PDI promotes thrombus formation in oxidative stress.
- PDI is a redox sensor that can function as either a reductase or an oxidase depending on the redox environment.
- Pharmacological approaches and PDI mutants were used to study the redox state's effect on thrombus formation.
- LOC14, which inhibits PDI reductase activity and induces oxidation, enhances thrombus formation.
- Blocking antibodies targeting PDI reversed the prothrombotic effects of LOC14.
- PDI mutants (C53A, C56A, R120D, T101A) were studied to understand sulfenylation-mediated oxidation.
- R120D mutant PDI failed to restore thrombus formation in oxidative stress conditions (GPx3-/- mice).
- PDI-catalyzed oxidation is crucial for thrombus formation in oxidative stress.