Fatty acid-induced lipid accumulation promotes radiosensitization in Huh7 hepatocellular carcinoma cells - PubMed
8 hours ago
- #lipid-metabolism
- #radiosensitization
- #hepatocellular-carcinoma
- Fatty acid (oleic acid) uptake increases lipid accumulation in Huh7 hepatocellular carcinoma cells without causing toxicity.
- Pretreatment with oleic acid enhances cell death when combined with X-ray irradiation, indicating radiosensitization.
- Increased lipid hydroperoxides and reactive oxygen species suggest oxidative stress mediates the radiosensitization effect.
- Oleic acid upregulates genes related to glutathione degradation and ferroptosis (ChaC glutathione specific γ-glutamylcyclotransferase 1).
- Antioxidant defense genes (glutathione peroxidase 4 and glutamate-cysteine ligase catalytic subunit) are also upregulated in response to treatment.
- The findings are based on an in vitro model, limiting clinical relevance and requiring further validation in vivo.