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Human serum albumin and biological fluids reduce cefiderocol activity in carbapenem-resistant Gram-negative bacteria - PubMed

5 hours ago
  • #Human Serum Albumin
  • #Cefiderocol
  • #Carbapenem-Resistant Pathogens
  • Human serum albumin (HSA) reduces cefiderocol (FDC) activity in carbapenem-resistant Gram-negative bacteria.
  • HSA exposure alters bacterial physiology, reducing biofilm formation and modulating β-lactam resistance genes.
  • FDC uptake relies on siderophore-mediated transport, and HSA's iron content affects bacterial susceptibility.
  • Time-kill assays show HSA attenuates FDC activity, allowing regrowth in multiple bacterial species.
  • Human biological fluids with high protein content similarly reduce FDC efficacy.
  • Transcriptional analyses indicate downregulation of iron uptake genes, affecting siderophore-dependent drug uptake.
  • Host proteins and fluid environments significantly modulate FDC activity, impacting treatment strategies.