Whole-protein screening and multi-modal profiling of antigen-specific CD4+ T cells at single-cell resolution - PubMed
3 hours ago
- #CD4+ T cells
- #Antigen Screening
- #Immunotherapy
- Development of a high-throughput platform for systematic whole-protein screening and multi-modal profiling of antigen-specific CD4+ T cells at single-cell resolution.
- Platform uses class II single-chain trimer libraries to detect antigen-specific CD4+ T cells while profiling antigen specificity, TCR sequences, MHC restriction, transcriptomes, and patient origins.
- Applied to SARS-CoV-2 spike receptor binding domain, identifying 2,188 antigen-specific CD4+ T cells and key immunogenicity metrics.
- Extended to HPV-16 E6/E7 screening in precancerous patients, identifying HPV-specific CD4 TCRs with therapeutic potential.
- Integrates high-throughput antigen screening with high-dimensional cellular characterization to guide vaccine design and TCR-based cancer immunotherapies.