A novel PEDV-specific linear B-cell epitope evades cross-reactivity with TGEV and PDCoV - PubMed
3 hours ago
- #B-cell epitope
- #PEDV
- #monoclonal antibody
- Monoclonal antibodies were developed against the PEDV nucleocapsid protein, identifying a novel linear B-cell epitope (272-ERDLKDIPEWR-282).
- The core residues essential for antibody recognition were pinpointed as Leu275, Lys276, Asp277, Ile278, Glu280, Trp281, and Arg282 through alanine scanning mutagenesis.
- Structural modeling suggests this epitope is exposed and adopts an α-helix and flexible loop conformation.
- Sequence alignments show low conservation of this epitope in other swine enteric coronaviruses like TGEV and PDCoV.
- Validation via indirect immunofluorescence assays and ELISA confirms mAb 1D1 specifically recognizes PEDV without cross-reactivity with TGEV or PDCoV.
- The findings highlight mAb 1D1 and its epitope as promising tools for differential diagnosis of swine enteric coronaviruses.