Linear RAG scanning mediates editing of Igκ variable region repertoires - PubMed
4 hours ago
- #RAG scanning
- #Igκ repertoire
- #V(D)J recombination
- Linear RAG scanning enables secondary editing of Igκ variable regions.
- Primary Vκ-to-Jκ1 rearrangements occur first, followed by secondary Vκ-to-Jκ2-5 rearrangements for repertoire expansion and central tolerance.
- Loop extrusion initially moves Vκs to the recombination centre for diffusional joining in primary rearrangements.
- Secondary rearrangements involve RAG scanning linearly from VκJκ1-based recombination centres across the Vκ locus.
- Cer/Sis deletion or displacement switches the mechanism from diffusional to linear scanning for secondary recombination.
- Strong Vκ-RSSs restrict secondary rearrangements to Vκs immediately upstream and support linear scanning-based inversional rearrangements.