Single-Cell transcriptomics unveils metabolic and cellular dysregulation in striae gravidarum - PubMed
3 days ago
- #striae gravidarum
- #single-cell transcriptomics
- #ACSBG1
- Striae gravidarum (SG) is characterized by disrupted dermal extracellular matrix (ECM) homeostasis, with fibroblast heterogeneity and metabolic dysregulation poorly understood.
- The study uses single-cell RNA sequencing (scRNA-seq) and single-nucleus RNA sequencing (snRNA-seq) on SG lesions and normal skin.
- SG skin shows significant cellular reorganization, with fibroblasts exhibiting profound transcriptional changes.
- A dysregulated sender-receiver axis between inflammatory sC7 and reparative sC5 fibroblast subsets is identified.
- Pseudotime analysis reveals blocked differentiation from sC7 to sC5, indicating disrupted cellular progression.
- The sC7 subset undergoes fatty acid metabolic reprogramming, marked by upregulation of ACSBG1 enzyme.
- Triacsin C treatment suppresses ECM gene expression and pro-fibrotic markers, partially restoring reparative transcriptional programs.
- Local Acsbg1 silencing in mice alleviates SG-like dermal remodeling, improving collagen and elastic fiber integrity.
- Targeting ACSBG1-mediated fatty acid metabolic reprogramming restores reparative transcriptional programs and ameliorates ECM disruption.
- ACSBG1 is identified as a potential therapeutic target for striae gravidarum.