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Neuronal APOE4-induced early hippocampal network hyperexcitability in Alzheimer's disease pathogenesis - PubMed

7 hours ago
  • #APOE4
  • #Alzheimer's Disease
  • #Hippocampal Hyperexcitability
  • APOE4 is the strongest genetic risk factor for Alzheimer's disease (AD), but its early impact on neuronal and network function is unclear.
  • Young APOE4 knockin mice show hippocampal network hyperexcitability, which predicts later cognitive deficits and originates from specific subpopulations of smaller, hyperexcitable neurons.
  • Removing neuronal APOE4 eliminates this early hyperexcitability, indicating a direct role of APOE4 in neuronal dysfunction.
  • Aged APOE4 knockin mice develop further excitability issues, including granule cell hyperexcitability and progressive inhibitory dysfunction in the dentate gyrus.
  • Single-nucleus RNA sequencing identifies age-dependent transcriptional changes and Nell2 as a candidate mediator of early hyperexcitability.
  • CRISPR interference knockdown of Nell2 rescues abnormal excitability, confirming its role in APOE4-driven dysfunction.
  • The study links neuronal APOE4-induced early network impairment to AD pathogenesis through molecular and circuit mechanisms.