A Potent CRISPR-Cas12l Double-Strand Break Gene Editor - PubMed
3 hours ago
- #Gene Editing
- #Double-Strand Break
- #CRISPR-Cas12l
- A new CRISPR-Cas12 endonuclease family, Cas12l, was discovered from the bacteria Armatimonadota.
- Cas12l is compact, recognizes a 5' C-rich protospacer adjacent motif, and has an N-terminal domain that locks the DNA target site.
- Engineered Asp2Cas12l variants showed a ~10-fold increase in double-strand break editing efficiency in human cells.
- Editing frequencies were comparable to SpCas9 at overlapping sites, with homology-directed repair correcting 39-56% of outcomes.
- The study enhances CRISPR-Cas12 protein engineering and provides a potent alternative for genome editing in human cells.