Identification of stable reference genes in plasma astrocyte-derived exosomal mRNA for RT-qPCR analysis in Alzheimer's disease - PubMed
5 hours ago
- #Alzheimer's disease
- #RT-qPCR
- #Exosomal mRNA
- Accurate RT-qPCR gene expression quantification requires stable reference genes for normalization.
- Astrocyte-derived exosomal mRNAs (ADEs) are promising biomarkers for Alzheimer's disease (AD) due to their stability and ability to cross the blood-brain barrier.
- The study evaluated reference gene stability in plasma ADEs across cognitively unimpaired (CU), mild cognitive impairment (MCI), and AD patients using five algorithms.
- YWHAE and RNPS1 were identified as the most stable reference genes across all groups, while GAPDH was found to be inadequate.
- This study provides the first systematic validation of reference genes for mRNA quantification in plasma ADEs, aiding AD biomarker development.