Myosin IIA motor regulates attaching-effacing bacteria interactions with intestinal epithelium - PubMed
5 hours ago
- #intestinal epithelium
- #actin cytoskeleton
- #Myosin II
- Attaching effacing (A/E) bacteria like EPEC and Citrobacter rodentium colonize intestinal epithelial cells (IECs) by remodeling the cytoskeleton and forming actin pedestals.
- Non-muscle myosin II (NM II) regulates the actin cytoskeleton, but its role in IEC colonization by A/E pathogens was unclear.
- Studies used mouse models with NM IIA deletion or mutation and NM IIC deletion, alongside in vitro experiments with IEC cell lines.
- NM IIA-deficient mice showed higher C. rodentium colonization, more severe inflammation, and crypt hyperplasia compared to controls.
- NM IIC deletion did not affect C. rodentium colonization.
- Blebbistatin (NM II inhibitor) increased EPEC attachment to IECs, while 4-HAP (NM IIC activator) had no effect.
- NM IIA knockout increased EPEC adhesion, requiring an intact bacterial Type 3 secretion system and Tir effector.
- NM IIA limits A/E pathogen colonization by inhibiting actin cytoskeleton remodeling induced by pathogens.