Characterization and engineering of highly efficient Cas12j genome editors - PubMed
5 hours ago
- #Cas12j
- #Genome Editing
- #CRISPR
- Cas12j nucleases are a hypercompact alternative to larger CRISPR-Cas enzymes but have modest editing efficiency.
- Eight novel Cas12j orthologs were identified from viral metagenomes, showing low editing activity in mammalian cells.
- Engineering T5 exonuclease-Cas12j fusions significantly enhanced genome-editing activity, comparable to established compact CRISPR-Cas editors.
- Robust cellular editing was observed with a previously unrecognized trinucleotide sequence context within the target DNA.
- Cas12j-based adenine base editors were developed, enabling efficient A-to-G base conversion in mammalian cells.
- The study expands the CRISPR toolbox by converting compact Cas12j nucleases into efficient genome-editing platforms for therapeutic applications.