Autophagy dysfunction in iPSCs-derived neurons and midbrain organoids carrying a SNCA triplication - PubMed
17 hours ago
- #Autophagy
- #Parkinson's Disease
- #iPSC Models
- Autophagy defects observed early in iPSC-derived neuronal cultures from PD patients with SNCA triplication.
- In midbrain organoids (hMOs) with SNCA triplication, autolysosome area decreased, while α-Synuclein levels increased by 50 days of differentiation.
- Autophagy impairment worsened by 70 days, correlating with dopaminergic neuron dysfunction and electrophysiological activity decline.
- Study supports using iPSC-derived models to link autophagy dysfunction, α-Synuclein pathology, and neurodegeneration in Parkinson's disease.
- Live-cell imaging with LC3-Rosella reporter enabled real-time assessment of autolysosome dynamics in human-relevant systems.