G Protein Gαq Subunits Engage Targets in the Nucleus Involved in Chromatin Remodeling and Gene Expression - PubMed
6 hours ago
- #Chromatin Remodeling
- #G Protein
- #Gene Expression
- Gαq is a critical mediator of cell and tissue responses to Gq-coupled receptor stimulation.
- A proximity labeling proteomic screen identified novel Gαq signaling partners, including nuclear proteins SMARCD3 (BAF60c) and BCAS2.
- Gαq interacts with SMARCD3 and BCAS2 in an activation-dependent manner, confirmed by luciferase complementation and pulldown experiments.
- A small portion of Gαq is present in the nucleus, increasing after GPCR activation or with an activating mutation.
- Proximity ligation assays show Gαq(Q209L) engages SMARCD3 in the nucleus, suggesting direct regulation of nuclear processes.