RIG-I-dependent sensing of PEDV shapes epithelial antiviral immunity in the intestinal mucosa - PubMed
5 hours ago
- #innate immunity
- #RIG-I
- #PEDV
- RIG-I is the dominant cytosolic sensor for PEDV in the intestinal mucosa, initiating interferon-mediated antiviral defense.
- A core RNA PAMP is located within nucleotides 375-760 of the 5' ORF1a region of the PEDV genome, directly engaged by RIG-I.
- Specific nucleotide motifs and cooperative stem-loop assemblies in the viral RNA structure are crucial for optimal RIG-I activation.
- This PEDV-derived RNA PAMP robustly activates RIG-I signaling, amplifies interferon and ISG responses, and restricts replication of PEDV and other RNA viruses.
- Co-administration of this viral RNA with an inactivated influenza vaccine enhances antigen-specific immunity and protection.
- The study provides insights into coronavirus sensing at mucosal surfaces and highlights viral RNA structures as potential immunostimulants for antiviral and vaccine strategies.