Amphiphilic Chitosan-PEI Hybrid Nanocarrier Enhances Delivery Efficiency and Immunogenicity of PEDV mRNA Vaccines - PubMed
5 hours ago
- #immunogenicity
- #mRNA vaccines
- #nanoparticles
- mRNA vaccines require efficient and safe delivery systems for robust antigen expression and immune activation.
- A novel chitosan derivative, PAN2H, was synthesized by grafting palmitic acid onto N-2-hydroxypropyl trimethyl ammonium chloride chitosan (N-2-HACC).
- mRNA-loaded nanoparticles (PEI/mRNA/PAN2H) were constructed by complexing mRNA with polyethyleneimine (PEI) and electrostatically binding to PAN2H.
- PEI/mRNA/PAN2H nanoparticles achieved approximately twofold higher expression of GFP- and Luc-mRNA in vitro compared to PEI-mediated transfection.
- In vivo, PEI/mRNA/PAN2H vaccination elicited significantly stronger antibody responses and cellular immune activation than the PEI control.
- The PAN2H-PEI hybrid strategy combines electrostatic condensation (PEI) with amphiphilicity-driven assembly and chitosan-associated biocompatibility/adjuvanticity (PAN2H).
- The cationic PEI/mRNA/PAN2H nanoparticles exhibit low cytotoxicity and markedly improved mRNA delivery capabilities.
- PAN2H is highlighted as a promising chitosan-derived platform for developing next-generation mRNA vaccines.