MitoPerturb-Seq identifies gene-specific single-cell responses to mitochondrial DNA depletion and heteroplasmy - PubMed
18 hours ago
- #Mitochondrial DNA
- #CRISPR Screening
- #Single-Cell Genomics
- MitoPerturb-Seq is a CRISPR-Cas9-based method for single-cell analysis of nuclear responses to changes in mtDNA.
- It studies how cells sense and control mtDNA copy number and heteroplasmy, linked to mitochondrial and neurodegenerative diseases.
- The research screened mtDNA maintenance genes in mouse cells with a heteroplasmic mt-Ta mutation.
- Findings include common and specific aspects of the integrated stress response to mtDNA depletion from Tfam, Opa1, and Polg knockout.
- Responses are partly ATF4-mediated and slow cell proliferation independently of cell-cycle stage.
- The method offers insights into mitochondrial-nuclear interactions and potential therapies for mitochondrial dysfunction.