Atomic-level architecture and assembly mechanism of high-order structures of RIPK1 fibril revealed by integrated structural biology - PubMed
7 hours ago
- #amyloid fibrils
- #structural biology
- #RIPK1
- RIPK1 forms higher-order fibril networks under slightly acidic conditions.
- The study used integrated structural biology techniques to reveal the atomic structure of mRIPK1 fibrils.
- The fibril core adopts an N-shaped parallel β-sheet conformation with dynamic regions aiding network formation.
- Electrostatic interactions between specific edge residues (D516-K519 or D537-K519) are crucial for network integrity.
- Site-directed mutagenesis confirmed the importance of these edge residues.
- The findings provide insights into hierarchical fibril assembly and potential for designing amyloid-based materials.