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Light-based modulation of astrocytic calcium for regulation of organelle dynamics and morphogenesis - PubMed

4 hours ago
  • #Organelle Dynamics
  • #Light-Based Modulation
  • #Astrocyte Calcium Signaling
  • Developed a tunable light-based tool to modulate astrocytic Ca2+ activity, mimicking endogenous signaling patterns.
  • Found that local Ca2+ spikes enhance organelle entry into processes, while global Ca2+ waves arrest organelle movement.
  • Showed that global Ca2+ elevations suppress motor-driven transport when bypassing cargo adaptors via FKBP-FRB assays.
  • Demonstrated that Ca2+-dependent distal organelle accumulation promotes astrocyte process elongation and structural remodeling.
  • The method provides a versatile platform for eliciting endogenous-like Ca2+ patterns and decoding their physiological roles in astrocytes.