Detection of Viruses of Public Health Importance in Wastewater Samples Using Conventional PCR Techniques and a Targeted Enrichment Whole Genome Sequencing Panel - PubMed
4 hours ago
- #Viral detection
- #Genomic sequencing
- #Wastewater-based epidemiology
- The study evaluated PCR/qPCR and targeted enrichment whole-genome sequencing (Viral Surveillance Panel, VSP) for detecting viruses in wastewater samples.
- Fifty-two wastewater samples from Córdoba, Argentina, were concentrated and pooled, with detection frequencies showing differences: Rotavirus A and Norovirus had 100% detection by PCR/qPCR but only 14.3% by VSP.
- VSP detected additional viruses like Astrovirus (71.4%) and enabled recovery of 16 near-complete genomes, providing broader genomic insights.
- PCR/qPCR was more sensitive and practical for key public health viruses, while VSP offered genomic data on understudied viruses, suggesting combined utility in surveillance.