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The exocyst subunits OsEXO70L2 and OsSEC3A regulate root development through modulating OsPIN1a/b-mediated auxin distribution in rice - PubMed

5 hours ago
  • #Exocyst Complex
  • #Auxin Distribution
  • #Root Development
  • The exocyst subunits OsEXO70L2 and OsSEC3A regulate root development in rice by modulating auxin distribution.
  • Mutations in OsEXO70L2 (sr1 mutant) and OsSEC3A (sec3a-1 mutant) result in shortened roots and reduced root apical meristem size.
  • OsEXO70L2 interacts with OsSEC3A, and they function in the same pathway, as shown by the sr1 sec3a-1 double mutant resembling sr1.
  • Both mutants exhibit reduced sensitivity to auxin (IAA) and auxin transport inhibitor (NPA), with disrupted auxin distribution in root tips.
  • OsEXO70L2 and OsSEC3A regulate the plasma membrane abundance and localization of auxin efflux carriers OsPIN1a/b.
  • The ospin1a ospin1b double mutant has more severe root defects, confirming OsPIN1a/b as downstream targets in this pathway.
  • The study concludes that OsEXO70L2 and OsSEC3A control root length via vesicle trafficking-dependent OsPIN1a/b localization and auxin distribution.