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Tubulin Acetylation Deficiency Promotes Axonemal Turnover and Increases Cytoplasmic Microtubules - PubMed

5 hours ago
  • #cilia regeneration
  • #microtubule dynamics
  • #tubulin acetylation
  • Tubulin acetylation deficiency was studied using a Chlamydomonas mutant lacking αTAT1 acetyltransferase.
  • Loss of acetylation increased axonemal tubulin turnover but did not affect steady-state lengths of axonemes and rootlets.
  • The mutant showed an increased number of dynamic cortical microtubules and could regenerate cilia without protein synthesis.
  • Increased cortical microtubules depended on the presence of cilia, as shown by an ift46-1 mutation abolishing ciliogenesis.
  • Cell growth and division remained normal despite cytoskeletal changes, suggesting cellular buffering against microtubule variations.