Three-dimensional genome reorganization foreshadows zygotic genome activation in Drosophila - PubMed
6 hours ago
- #3D genome organization
- #Drosophila embryogenesis
- #Zygotic genome activation
- Pico-C, a low-input Micro-C approach, enables high-resolution 3D genome mapping during early Drosophila embryogenesis.
- Contrary to previous beliefs, the genome is dynamically organized before zygotic genome activation (ZGA), with chromatin loops emerging during pre-ZGA nuclear cycles.
- Spatial autocorrelation analysis reveals context-dependent regulatory influences on chromatin.
- Inhibition of transcriptional elongation has site-specific effects, retaining some early loops while weakening insulation at active promoters.
- Machine learning models identify motif-specific contributions to genome architecture.
- Co-depletion of pioneer factors Zelda and GAF leads to factor-specific perturbations in chromatin architecture.
- Early genome organization is orchestrated by overlapping yet separable regulatory inputs.