Ana1/CEP295 regulates centriolar doublet-to-triplet conversion during spermatogenesis - PubMed
4 hours ago
- #Microtubule Dynamics
- #Spermatogenesis
- #Centriole
- The study investigates Ana1/CEP295's role in regulating the conversion of microtubule doublets to triplets in centrioles during Drosophila spermatogenesis.
- In the absence of Ana1, the conversion process is impaired. The N-terminal region of Ana1 localizes near microtubule doublets and promotes elongation, while the C-terminal region supports C-tubule assembly.
- Ana1 recruits centrobin to centrioles via its C-terminal region, and targeted recruitment of centrobin can restore C-tubule formation even without Ana1.
- Restoring Ana1 expression at different developmental stages shows that triplet microtubule integrity is crucial for male fertility, highlighting the biological significance of this conversion process.
- The findings identify Ana1 as a key regulator of centriolar doublet-to-triplet conversion and provide insights into its evolutionary and functional importance.