O-GlcNAc transferase orchestrates oocyte maturation by modulating the activity of mitochondrial respiratory chain complex I - PubMed
5 hours ago
- #mitochondrial function
- #female fertility
- #O-GlcNAcylation
- O-GlcNAc transferase (OGT) is crucial for oocyte maturation and female fertility.
- OGT knockout in mouse oocytes leads to infertility, impaired oocyte maturation, and defective follicle development.
- OGT deficiency disrupts kinetochore-microtubule attachments, activating the spindle assembly checkpoint and causing meiotic arrest.
- Multi-omics analysis shows Ogt knockout impairs mitochondrial respiratory chain complex I function and disrupts NADH-to-NAD+ conversion.
- OGT interacts with mitochondrial complex I subunit NDUFA8; its knockout reduces NDUFA8 levels, contributing to mitochondrial dysfunction.
- OGT depletion causes mitochondrial dysfunction, including abnormal distribution, diminished membrane potential, elevated oxidative stress, and reduced ATP production.