A selective enrichment and specific probe terminal mediated strategy for highly sensitive detection of microRNAs - PubMed
6 hours ago
- #diagnostic accuracy
- #liquid biopsy
- #microRNA detection
- SE-SPTM-PCR is a new detection platform for microRNAs combining selective enrichment with specific probe terminal mediated RT-qPCR.
- The method eliminates nonspecific amplification and achieves 100-fold higher sensitivity than conventional stem-loop RT-qPCR.
- Clinical studies show improved diagnostic accuracy for colorectal cancer, HCMV reactivation, and nasopharyngeal carcinoma.
- SE-SPTM-PCR enhances the performance of hsa-miR-92a-3p for colorectal cancer detection, increasing AUC from 0.72 to 0.85.
- The platform restores utility to abandoned biomarkers like hcmv-miR-UL22A-5p and ebv-miR-BART3-3p, achieving high AUC values.
- SE-SPTM-PCR offers a robust tool for miRNA-based liquid biopsy, supporting early disease detection and personalized treatment.