Mapping cellular heterogeneity and dynamic interactions in pancreatic cancer - PubMed
12 hours ago
- #Pancreatic Cancer
- #Single-cell RNA-seq
- #Biomarkers
- Pancreatic ductal adenocarcinoma (PDAC) is highly aggressive with a high mortality rate.
- Intra-tumoral heterogeneity (ITH) increases PDAC severity and complicates treatment.
- Single-cell RNA sequencing (scRNA-seq) provides spatially resolved transcriptomic profiling for PDAC research.
- The review discusses sample preparation, library preparation, data analysis, and challenges of scRNA-seq.
- scRNA-seq helps identify dynamic cell subset transformations, biomarkers, and therapeutic vulnerabilities in PDAC.
- Biomarkers like MMP1 and S100A2+ tumor subsets are identified through scRNA-seq.
- Cancer-associated fibroblasts and therapeutic inhibitors show potential in preventing PDAC resistance.
- The roles of immune cells, metabolic reprogramming, autophagy, and apoptosis in PDAC are explored.