Structural polymorphism of ex-vivo ALECT2 amyloid fibrils revealed by cryo-EM - PubMed
5 hours ago
- #ALECT2 amyloidosis
- #cryo-electron microscopy
- #amyloid fibril polymorphism
- Cryo-EM structures reveal structural polymorphism in ex-vivo ALECT2 amyloid fibrils from a patient's kidney, with a predominant single-protofilament and two minor double-protofilament morphologies.
- The dominant fibril comprises full-length 133-residue LECT2 protein, retaining all three native disulfide bonds, while double-protofilament forms show similar folds but different inter-filament interfaces.
- Mass spectrometry identified acetylation within the fibrils, providing molecular insights that could aid in diagnostic and therapeutic development for ALECT2 amyloidosis.