Tobacco exposure and peripheral artery disease: A multi-omics investigation integrating epidemiology, genetics, toxicology, and single-cell transcriptomics - PubMed
6 hours ago
- #Peripheral artery disease
- #Multi-omics
- #Tobacco exposure
- Elevated serum cotinine levels, a biomarker of tobacco exposure, were linearly associated with increased peripheral artery disease (PAD) prevalence in epidemiological analyses.
- Network toxicology and Mendelian randomization identified IL6 and STAT3 as inflammation-related mediators with genetically predicted effects on PAD susceptibility.
- Molecular docking and dynamics suggested potential transient interactions between cotinine and IL6/STAT3, providing hypothesis-generating insights.
- Single-cell RNA sequencing revealed IL6 and STAT3 expression in vascular-associated immune and stromal cell populations, offering cellular context for cotinine-related inflammation.
- The findings suggest IL6/STAT3 signaling may represent a cotinine-related pathway potentially mediating the link between tobacco exposure and PAD, highlighting public health priorities for reducing tobacco exposure.