Human DHX29 detects nonoptimal codon usage to regulate mRNA stability - PubMed
5 hours ago
- #gene expression
- #translation regulation
- #mRNA stability
- Synonymous codon usage affects gene expression in prokaryotes and eukaryotes.
- Nonoptimal codons lead to mRNA decay, but the mechanism in human cells is unclear.
- CRISPR screening identified DHX29 as a key regulator of codon-dependent gene expression.
- Cryo-EM and ribosome profiling show DHX29 interacts with the A-site entrance of the 80S ribosome.
- DHX29 monitors aminoacyl-tRNA sampling during translation.
- Proteomic analysis reveals DHX29 recruits the GIGYF2•4EHP complex to suppress nonoptimal mRNAs.
- The study links synonymous codon usage to gene expression regulation.