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Niemann-Pick C1 maintains cholesterol homeostasis in late endosomes to drive hepatitis B virus endosomal escape - PubMed

3 days ago
  • #cholesterol homeostasis
  • #HBV entry
  • #endosomal trafficking
  • NPC1, a late endosomal cholesterol transporter, is a novel host factor promoting HBV escape into the cytoplasm by maintaining cholesterol homeostasis in late endosomes.
  • NPC1 knockdown significantly reduces HBV susceptibility and causes viral retention in late endosomes with cholesterol accumulation, without impairing transcription or replication.
  • Cholesterol transport-defective NPC1 mutant fails to restore infection, while cholesterol efflux rescues viral release, and pharmacological NPC1 inhibition potently blocks HBV infection.