Qingjin Pingchuan formula attenuates pulmonary inflammation by reprogramming neutrophil SHP1-JAK2/SRC-STAT3 signaling - PubMed
5 hours ago
- #Pulmonary Inflammation
- #Neutrophil Signaling
- #Traditional Chinese Medicine
- Qingjin Pingchuan Formula (QJPC) is a traditional Chinese medicine used for severe community-acquired pneumonia (SCAP).
- The study explores QJPC's anti-inflammatory effects via neutrophil-mediated SHP1-JAK2/SRC-STAT3 signaling.
- UPLC-MS identified 17 systemically absorbed constituents in QJPC.
- Network pharmacology and molecular docking predicted JAK2, SRC, and STAT3 as key targets.
- QJPC improved lung histopathology, reduced IL-6, and increased IL-10 in LPS-injured mice.
- Single-cell RNA sequencing showed QJPC reshapes neutrophil heterogeneity and upregulates SHP1 (Ptpn6).
- QJPC enhances SHP1 expression, suppresses STAT3 activation, and attenuates JAK2/SRC signaling in neutrophils.
- Neutrophil-depleted mice showed diminished anti-inflammatory effects of QJPC.
- SHP1 agonist SC43 co-treatment modulated QJPC's effects, confirming SHP1's role.
- QJPC's lung-protective effects involve SHP1 upregulation and JAK2/SRC-STAT3 pathway inhibition in neutrophils.