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Proteinase 3 Drives Murine Diabetic Kidney Disease by Mediating Caspase-3-dependent Apoptosis of Podocytes - PubMed

6 hours ago
  • #Diabetic Kidney Disease
  • #Proteinase 3
  • #Podocyte Apoptosis
  • Proteinase 3 (PR3) is identified as a key mediator in murine diabetic kidney disease (DKD), driving podocyte apoptosis via caspase-3 activation.
  • Podocyte-specific PR3 knockout mice showed reduced proteinuria, mesangial matrix expansion, and podocyte injury in DKD models.
  • High glucose conditions increased PR3 levels and activity in podocytes, leading to caspase-3 cleavage and apoptosis, which was mitigated by PR3 inhibition.
  • Overexpression of PR3 in podocytes exacerbated caspase-3 cleavage and apoptosis, while PR3 deficiency protected against podocyte injury.
  • Therapeutic intervention with elafin, a serine protease inhibitor, attenuated podocyte loss and DKD progression in mice, suggesting a potential treatment strategy.