Targeted blocking of gene splicing can dysregulate intron-embedded primary microRNAs - PubMed
6 days ago
- #microRNA
- #splicing
- #ASO
- Antisense oligonucleotides (ASOs) targeting gene splicing can affect intron-embedded noncoding RNAs (ncRNAs).
- ASO targeting slit3 splice site disrupts pituitary axonal morphogenesis in zebrafish.
- Increased expression of slit3, mir-218a-1, and related genes observed with ASO treatment.
- Phenotype is dependent on mir-218a-1, as blocking mature mir-218a-1 or using mutants prevents the effect.
- Intron-retained transcripts can escape nonsense-mediated decay (NMD) and increase intronic primary microRNA expression.
- Findings suggest a need for further validation of ASO effects on other ncRNAs.