Spatio-temporal dynamics of autophagy-associated genes in macrophage-driven atherosclerosis: an integrated omics and experimental study - PubMed
6 hours ago
- #macrophage
- #autophagy
- #atherosclerosis
- Identified three autophagy-related genes (SNX5, SMG1, GSK3A) as core regulators in macrophage-driven atherosclerosis.
- Used multi-omics approach including transcriptomic datasets, single-cell RNA-seq, and spatial transcriptomic mapping.
- Demonstrated strong diagnostic potential for atherosclerosis (combined AUC = 0.844).
- Linked genes to immune cell infiltration, metabolic reprogramming, and immune-inflammatory pathways like NF-κB.
- Single-cell and spatial analysis revealed distinct expression patterns across plaque regions and cell types.
- In vitro experiments confirmed upregulation of SNX5, SMG1, and GSK3A upon ox-LDL induction in macrophages.
- Genes show dynamic spatiotemporal expression during macrophage-to-foam cell transition.