Bacteroides-associated NAD⁺ depletion correlates with exacerbated radiation-induced colorectal injury and impaired mucosal proliferative capacity - PubMed
2 days ago
- #NAD metabolism
- #Bacteroides
- #Radiation proctitis
- Radiation proctitis (RP) is a common complication of pelvic radiotherapy affecting treatment and quality of life.
- Severe RP cases showed enrichment of Bacteroidales, while mild cases had more Firmicutes in fecal microbiomes.
- Severe RP was linked to reduced NAD�� levels and increased microbial nicotinate/nicotinamide metabolism genes, particularly from Bacteroides species.
- Fecal microbiota transplantation from severe-RP donors worsened radiation-induced colorectal injury in mice, lowering NAD�� levels.
- Bacteroides gavage in mice worsened pathology, reduced mitochondrial membrane potential, and decreased Lgr5�� stem-cell proportion and proliferative indices.
- Nicotinamide mononucleotide (NMN) supplementation attenuated injury and restored NAD�� levels, reversing Bacteroides-induced effects.
- The study suggests Bacteroidales enrichment correlates with NAD�� depletion and exacerbated radiation-induced colorectal injury, offering potential microbiome- and metabolite-targeted therapies.