Smart microenvironment-adaptive nanocatalytic hydrogel for sequential antibacterial, anti-inflammatory, and regenerative therapy of biofilm-infected wounds - PubMed
5 hours ago
- #wound healing
- #nanocatalytic hydrogel
- #sequential therapy
- A smart hydrogel dressing (HCOC) integrates humic acid-encapsulated copper nanozymes (Cu5.4O) into an alginate-chitosan network for biofilm-infected wound therapy.
- In acidic biofilm phases (pH < 6.5), humic acid aggregates release Cu5.4O for chemodynamic therapy (CDT) and enables photothermal therapy (PTT), achieving >99.99% antibacterial efficacy and dispersing 87.46% of biofilms.
- As wound pH rises post-infection (pH ≥ 7.0), humic acid dissolves to release more Cu5.4O nanozymes, which switch to antioxidant modes, scavenging >90% of reactive oxygen species and promoting M2 macrophage polarization via NF-κB and Wnt/β-catenin signaling.
- In vivo, HCOC with NIR irradiation accelerates wound healing with 91.65% closure in 7 days, enhancing angiogenesis (∼90 CD31+ cells/field) and M2 macrophage infiltration (∼110 CD163+ cells/field).
- This platform offers microenvironment-responsive sequential therapy for precision wound management, targeting antibacterial, anti-inflammatory, and regenerative phases.