Single-cell and spatial transcriptomics define 20E-driven developmental reprogramming in silkworm wing disc - PubMed
4 hours ago
- #hormone signaling
- #developmental biology
- #transcriptomics
- Single-cell and spatial transcriptomics used to study silkworm wing disc development.
- Identified 12 major cell types and their developmental transitions in the wing disc.
- Wing morphogenesis (Wm) cells act as central progenitors differentiating into epithelial and cuticle lineages.
- Time-resolved snRNA-seq reveals hierarchical transcriptional reprogramming with Wm cells as early signaling hubs.
- 20-hydroxyecdysone treatment accelerates fate transitions and gene expression, mimicking natural development.
- A five-stage Gene Transition Model proposed for progressive fate resolution in wing development.
- Provides insights into hormone-driven organogenesis and potential agricultural applications.