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Self-amplifying RNA therapy encoding CNTF with disulfiram co-delivery promotes optic nerve repair through microglial pyroptosis inhibition and RGC axonal regeneration - PubMed

5 hours ago
  • #optic nerve repair
  • #RNA therapy
  • #neuroinflammation
  • Self-amplifying RNA therapy combined with disulfiram co-delivery promotes optic nerve repair.
  • The therapy targets microglial pyroptosis inhibition and RGC axonal regeneration.
  • Traumatic optic neuropathy (TON) causes irreversible vision loss with no current effective treatment.
  • Microglial pyroptosis via the NLRP3/CASP1/GSDMD pathway exacerbates RGC degeneration.
  • Engineered lipid nanoparticles (LNPs) deliver disulfiram and saRNA encoding CNTF.
  • The combinatorial strategy suppresses neuroinflammation and provides neurotrophic support.
  • Results show enhanced RGC survival, axonal regeneration, and visual function restoration.
  • The approach is a promising multi-target treatment for neuroinflammatory and neurodegenerative diseases.