Simultaneous spatial transcriptomics and morphology profiling as tools to explore how microglia change with age - PubMed
14 hours ago
- #aging
- #microglia
- #spatial transcriptomics
- Combined multiplexed error-robust fluorescence in situ hybridization with immunohistochemistry to study microglia in young and aged mouse brains.
- Revealed that mRNA spatial organization varies across microglial states, showing distinct localization patterns within their processes.
- Identified a subpopulation of disease-associated-like microglia with ramified morphology, challenging conventional assumptions about morphology and microglial states.
- Found that aging reshapes mRNA distributions and co-localization networks, shifting microglial programs towards migration and catabolic regulation.
- Highlights the role of subcellular transcript organization in shaping microglial morphology and function, offering new research avenues in health, disease, and aging.