Modifying meiotic recombination by targeting chromatin regulators to crossover hotspots in Arabidopsis - PubMed
5 hours ago
- #chromatin regulators
- #Arabidopsis
- #meiotic recombination
- Study uses dCas9-based system to recruit histone demethylase JMJ14 to specific genomic loci in Arabidopsis.
- Recruitment of JMJ14 reduces H3K4me3 levels and decreases crossover frequency in targeted regions.
- Observed reduced expression of a long noncoding RNA (lncRNA) and altered crossover topology at the hotspot.
- Suppressed recombination also noted at neighboring, untargeted hotspots.
- Targeting transcriptional activator VP64 to the same region increases lncRNA expression and crossover frequency.
- Establishes causal link between H3K4me3, transcription, and local crossover activity.
- H3K4me3 levels are closely associated with transcriptional output and recombination frequency.