M2pep-modified liposomal nanoparticles delivering siITGB4 induce apoptosis and inhibit NSCLC metastasis via macrophage reprogramming - PubMed
12 hours ago
- #NSCLC
- #MacrophageReprogramming
- #Nanomedicine
- M2pep-modified liposomal nanoparticles (M2pep-LNP@siITGB4) deliver siRNA targeting integrin β4 (siITGB4) to reprogram M2 tumor-associated macrophages (TAMs) and inhibit NSCLC metastasis.
- The nanoparticles downregulate M2 markers (CD206, Arg1, IL-10) and upregulate M1 markers (CD86, iNOS), enhancing CD8+ T cell infiltration.
- Silencing ITGB4 reduces GNB5 expression and FAK/Src/AKT phosphorylation, promoting apoptosis and inhibiting epithelial-mesenchymal transition (EMT).
- RNA sequencing reveals 3494 differentially expressed genes, with suppressed ECM-receptor interactions.
- In vivo studies show significant reduction in tumor volumes and metastatic lesions.
- This approach offers a novel nanomedicine-based strategy for enhancing anti-tumor immunity in NSCLC.