Cas9+ conditionally immortalized neutrophil progenitors as a tool for genome wide CRISPR screening for neutrophil differentiation and function - PubMed
6 hours ago
- #CRISPR Screening
- #Neutrophil Differentiation
- #Genetic Tools
- Developed a Cas9+ER-Hoxb8 neutrophil progenitor cell line enabling genetic analysis of neutrophils.
- The cell line allows efficient production of Cas9-edited neutrophils by withdrawing estrogen after sgRNA transduction.
- Neutrophil differentiation occurs both in vitro and when transferred into mice, supporting in vivo studies.
- A pooled CRISPR screen identified hundreds of genes required for survival during neutrophil differentiation, including Cebpe.
- Validated Cebpe's role in neutrophil differentiation in vivo and identified the WASH complex as essential for development.
- This tool facilitates genome-wide CRISPR screens to study neutrophil differentiation and function in disease contexts.