Rooting Conifer Genetic Research: An Accessible and Efficient Transformation System - PubMed
5 hours ago
- #genetic-transformation
- #conifers
- #long-distance-signaling
- Conifers are crucial to global forest ecosystems but face challenges in genetic transformation.
- Researchers engineered a binary vector with chimeric Ri plasmid T-DNA borders, VirGN54D, and pVS1 R106H to overcome transformation resistance.
- The optimized system enables efficient, cross-species genetic transformation, including in gymnosperms like Ginkgo biloba.
- A non-aseptic root transformation system was established for Pinus tabuliformis.
- The system allows study of functional genomics and evolutionary mechanisms, such as long-distance macromolecular trafficking.
- Transgenic deployment of Arabidopsis florigen FT in Chinese pine roots showed conserved protein mobility despite lacking FT orthologs.
- Conifer age biomarker DAL1 showed no mobility even with TLS tag, revealing lineage-specific mRNA transport constraints.
- The work provides a versatile genetic tool for challenging plant species and insights into long-distance signaling evolution.
- Streamlined protocols and universal vector system address bottlenecks in conifer biotechnology and non-model plant genomics.