Pharmacological and genetic modulation of IL-32 expression in intestinal epithelial cells does not impact HIV-1 outgrowth in co-cultured CD4+ T-cells - PubMed
5 hours ago
- #IL-32 modulation
- #HIV-1 latency
- #intestinal epithelial cells
- The study investigated whether modulating IL-32 expression in intestinal epithelial cells (IECs) affects HIV-1 outgrowth from CD4+ T-cells in co-culture.
- IL-22 and rosiglitazone (RGZ) increased IL-32 expression in TNF-activated IECs, while all-trans retinoic acid (ATRA) decreased it, and IL-26 had no effect.
- Pharmacological treatments (IL-22, RGZ, ATRA) altering IL-32 levels in IECs did not impact HIV-1 outgrowth from co-cultured CD4+ T-cells of ART-treated people with HIV.
- Genetic knockout of IL-32 in IECs using CRISPR/Cas9 did not significantly change transcriptional profiles or affect HIV-1 outgrowth compared to control IECs.
- The findings suggest that IL-32 modulation in IECs does not play a major role in HIV-1 latency or reactivation, supporting its potential as a therapeutic target for reducing comorbidities in HIV patients.