BCDX2-CX3 and DX2-CX3 complexes assemble and stabilize RAD51 filaments - PubMed
5 hours ago
- #Homologous recombination
- #DNA repair
- #RAD51
- BCDX2-CX3 and DX2-CX3 complexes assemble and stabilize RAD51 filaments, crucial for DNA double-strand break repair via homologous recombination (HR).
- The study reveals a novel BCDX2-CX3-RAD51 supercomplex, where CX3 stacks atop BCDX2, forming a protofilament template for RAD51 filament assembly.
- A RAD51B-independent DX2-CX3 complex is identified, functioning as a stable RAD51 anchor on single-stranded DNA (ssDNA), capable of capping RAD51 filament segments.
- The complexes exhibit differential regulation by ATPase activity, with BCDX2-CX3 acting as a dynamic 'loader' and DX2-CX3 as a stable 'anchor', providing modularity to HR machinery.
- The findings offer a mechanistic understanding of RAD51 paralog function and a framework for interpreting mutations linked to cancer and genetic disorders.