A Lamp2a-linked RNA secreted by ADSCs prevents ENO1-lactylation-glycolysis feedback and cell malignant behavior in triple-negative breast cancer - PubMed
6 hours ago
- #Glycolysis feedback
- #Triple-negative breast cancer
- #ENO1 lactylation
- Triple-negative breast cancer (TNBC) lacks common breast cancer receptors and is linked to a hypoxic tumor microenvironment.
- ENO1 protein lactylation in BC samples was identified via 4D label-free lactylation quantitative proteomics analysis.
- Lactylation increases ENO1 protein stability and enzyme activity, promoting glycolysis and establishing a positive feedback loop.
- This feedback mechanism helps TNBC cells adapt to hypoxia and enhances malignant behaviors.
- Lactylation prevents lysosomal degradation of ENO1.
- An RNA ligand bound by ENO1 was identified to interfere with its lactylation.
- The RNA ligand is linked to Lamp2a protein in adipose stem cells (ADSCs) and secreted via exosomes.
- The Lamp2a-linked RNA ligand induces lysosomal degradation of ENO1 in TNBC cells, inhibiting glycolysis and malignant behaviors.
- The study provides a strategy for targeting ENO1 in TNBC treatment.