Quantification and transcriptome profiling reveal abundant, dynamic and translatable dephospho-CoA-capped RNAs - PubMed
12 hours ago
- #Transcriptomics
- #Gene expression
- #RNA capping
- Dephospho-CoA (dpCoA) is a noncanonical RNA cap that remains understudied due to detection limitations.
- Arabidopsis NUDT11 was identified as a specific decapping enzyme for dpCoA-RNA.
- New biochemical and transcriptomic methods were developed to quantify and profile dpCoA-RNA across species.
- dpCoA-RNAs exhibit tissue-specific and condition-specific variations and respond rapidly to high light intensity.
- In Arabidopsis, dpCoA-RNAs can reach up to 15% of m7G-capped RNAs and are associated with translating ribosomes.
- In vitro transcribed dpCoA-RNA was shown to be translatable in human cells.
- The study highlights the dynamic role of dpCoA caps in gene expression regulation.